| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
140 μL bronchoalveolar lavage fluid samples (WH01 to WH04) were reserved for RNA extraction using the QIAamp Viral RNA Mini Kit (52904; Qiagen, Heiden, Germany), according to the manufacturer's recommendations. A probe-captured technique was used to remove human nucleic acid. The remaining RNA was reverse-transcribed into cDNA, followed by the second-strand synthesis. Using the synthetic double-stranded DNA, a DNA library was constructed through DNA-fragmentation, end-repair, adaptor-ligation, and PCR amplification. The constructed library was qualified with an Invitrogen Qubit 2.0 Fluorometer (ThermoFisher, Foster City, CA, USA), and the qualified double-stranded DNA library was transformed into a single-stranded circular DNA library through DNA-denaturation and circularisation. DNA nanoballs were generated from single-stranded circular DNA by rolling circle amplification, then qualified with Qubit 2.0 and loaded onto the flow cell and sequenced with PE100 on the DNBSEQ-T7 platform (MGI, Shenzhen, China). |
OTHER |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR456598 |
CRR456598_f1.fastq.gz
CRR456598_r2.fastq.gz
|
15,055.76
19,536.06
|
| CRR456599 |
CRR456599_f1.fastq.gz
CRR456599_r2.fastq.gz
|
24,939.11
29,552.37
|
| CRR456600 |
CRR456600_f1.fastq.gz
CRR456600_r2.fastq.gz
|
29,667.03
37,813.13
|
| CRR456601 |
CRR456601_f1.fastq.gz
CRR456601_r2.fastq.gz
|
17,070.75
20,872.05
|
|
