| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
140 μL bronchoalveolar lavage fluid samples (WH01 to WH04) were reserved for RNA extraction using the QIAamp Viral RNA Mini Kit (52904; Qiagen, Heiden, Germany), according to the manufacturer's recommendations. A probe-captured technique was used to remove human nucleic acid. The remaining RNA was reverse-transcribed into cDNA, followed by the second-strand synthesis. Using the synthetic double-stranded DNA, a DNA library was constructed through DNA-fragmentation, end-repair, adaptor-ligation, and PCR amplification. The constructed library was qualified with an Invitrogen Qubit 2.0 Fluorometer (ThermoFisher, Foster City, CA, USA), and the qualified double-stranded DNA library was transformed into a single-stranded circular DNA library through DNA-denaturation and circularisation. DNA nanoballs were generated from single-stranded circular DNA by rolling circle amplification, then qualified with Qubit 2.0 and loaded onto the flow cell and sequenced with PE100 on the DNBSEQ-T7 platform (MGI, Shenzhen, China). |
OTHER |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR456602 |
CRR456602_f1.fastq.gz
CRR456602_r2.fastq.gz
|
22,911.63
26,183.44
|
| CRR456603 |
CRR456603_f1.fastq.gz
CRR456603_r2.fastq.gz
|
19,824.75
21,976.34
|
| CRR456604 |
CRR456604_f1.fastq.gz
CRR456604_r2.fastq.gz
|
5,870.6
6,532.55
|
| CRR456605 |
CRR456605_f1.fastq.gz
CRR456605_r2.fastq.gz
|
19,536.97
21,843.93
|
|
