| 实验编号 | CRX457816 |
| 物种名称 | Xenopus |
| 标题 | TsLe-s11-2 |
| 项目编号 | PRJCA009859 |
| 样本编号 | SAMC803132 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA was extracted using TransZol Up lysis reagent. RNA-seq libraries were prepared using the VAHTS® Universal V6 RNA-seq Library Prep for Illumina Kit following the manufacturer's instructions. Briefly, mRNA Capture Beads were added to 2 μg total RNA samples for mRNA isolation by incubation at room temperature. Isolated mRNA samples were randomly fragmented into 150-200 bp fragments by adding Frag/primer Buffer and incubating at 94°C for 8 min. Double stranded cDNA was synthesized, ligated with an adapter, and purified using 0.45× DNA Clean Beads. Library amplification was performed using the purified products as templates. The PCR products were purified using DNA Clean Beads. Library quality was analyzed using an Agilent Technologies 2100 Bioanalyzer. Sequencing of the Llibraries was performed on the NovaSeq 6000. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2023-05-06 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR517846 |
CRR517846_f1.fq.gz
CRR517846_r2.fq.gz
|
1,264.62
1,343.23
|
|
| 提交者 | Hao Jiang (john-jh@foxmail.com) |
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| 所属单位 | Southern University of Science and Technology |
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| 提交日期 | 2022-06-20 |
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