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Experiment information
Accession CRX467654
Organism Mus musculus
Title OC_8
BioProject PRJCA009794
BioSample SAMC814932
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
Mice stool samples were collected for bacterial 16S rRNA gene sequencing. For microbiome analysis, fecal pellets were immediately collected after anatomy and snap-frozen on dry ice, then stored at -80 °C until sequencing. Microbial community genomic DNA was extracted from stool samples using the E.Z.N.A.® soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to manufacturer’s instructions. The DNA extract was checked on 1% agarose gel, and DNA concentration and purity were determined with NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, USA). The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') by an ABI GeneAmp® 9700 PCR thermocycler (ABI, CA, USA). AMPLICON OTHER PCR PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Release date2023-01-06
Run
Run accession Run data file information
File nameFile size (MB)
CRR527776 CRR527776_f1.fastq.gz
CRR527776_r2.fastq.gz
8.13
12.16
SubmitterGuanghui Liu (ghliu@ioz.ac.cn)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2022-07-07
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