| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
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Crop tissue were pulverized, and 37% formaldehyde was added to obtain a final concentration of 4% for chromatin cross-linking. Mixture were incubated at room temperature (20-25°C) for 30 minutes, then glycine was added to obtain a final concentration of 0.25 mol/L to quench the formaldehyde. The mixture was then centrifuge at 1,500×g for 10 min at room temperature, and sediment was added the lysis buffer and homogenized. Homogenate was centrifuge at 5,000×g for adipocytes sediment. Nuclei of formaldehyde fixed adipose tissue were permeabilized and DNA was digested with 200 units of Dpn II (a 4-cutter restriction enzyme) for one hour at 37°C. The restriction fragment overhangs were filled and labbled by biotinylated nucleotides, then ligated in a small volume. After crosslink. reversal, ligated DNA was purified and sheared to a length of 300-500 bp, at which point ligation junctions were pulled down with streptavidin beads and prepped for BGISEQ-500 sequencing. |
Hi-C |
GENOMIC |
RANDOM PCR |
PAIRED
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