| Accession | CRX463650 |
| Organism | Columba livia |
| Title | Nur_1day_Hi-C_replicate_2 |
| BioProject | PRJCA010163 |
| BioSample | SAMC810521 |
| Platform | BGISEQ-500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Crop tissue were pulverized, and 37% formaldehyde was added to obtain a final concentration of 4% for chromatin cross-linking. Mixture were incubated at room temperature (20-25°C) for 30 minutes, then glycine was added to obtain a final concentration of 0.25 mol/L to quench the formaldehyde. The mixture was then centrifuge at 1,500×g for 10 min at room temperature, and sediment was added the lysis buffer and homogenized. Homogenate was centrifuge at 5,000×g for adipocytes sediment. Nuclei of formaldehyde fixed adipose tissue were permeabilized and DNA was digested with 200 units of Dpn II (a 4-cutter restriction enzyme) for one hour at 37°C. The restriction fragment overhangs were filled and labbled by biotinylated nucleotides, then ligated in a small volume. After crosslink. reversal, ligated DNA was purified and sheared to a length of 300-500 bp, at which point ligation junctions were pulled down with streptavidin beads and prepped for BGISEQ-500 sequencing. |
Hi-C |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
|
| Release date | 2023-02-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR523727 |
CRR523727_f1.fq.gz
CRR523727_r2.fq.gz
|
101,055.22
103,556.24
|
|
| Submitter | Xun Wang (xunwang@sicau.edu.cn) |
|---|
| Organization | sichuan agricultrural university |
|---|
| Date submitted | 2022-06-26 |
|---|
