| Accession | CRX499894 |
| Organism | Mus musculus |
| Title | WT-1-RA-24h-H3K27ac-chip-seq |
| BioProject | PRJCA011582 |
| BioSample | SAMC867615 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
cells were cross-linked with 1% formaldehyde. Sonication was carried out to shear the chromatin to yield DNA fragment sizes of 100 to 500 base pairs (bp). Samples were cleared and precleared for 1 hour with 40 μl of protein A/G agarose beads. Part of the precleared samples was used as input, and the remainder was immunoprecipitated with H3K27ac and H3K4me3 antibody and protein A/G agarose beads. The precipitated DNA and input DNA were sequenced using an Illumina sequencer. |
ChIP-Seq |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-09-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR563843 |
CRR563843_f1.fastq.gz
CRR563843_r2.fastq.gz
|
1,101.79
1,151.26
|
|
| Submitter | Wenbin Wang (sciencedb@gibh.ac.cn) |
|---|
| Organization | Nankai University |
|---|
| Date submitted | 2022-08-31 |
|---|
