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Experiment information
Accession CRX499897
Organism Mus musculus
Title WT-2-RA-24h-H3K4me3-chip-seq
BioProject PRJCA011582
BioSample SAMC867618
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
cells were cross-linked with 1% formaldehyde. Sonication was carried out to shear the chromatin to yield DNA fragment sizes of 100 to 500 base pairs (bp). Samples were cleared and precleared for 1 hour with 40 μl of protein A/G agarose beads. Part of the precleared samples was used as input, and the remainder was immunoprecipitated with H3K27ac and H3K4me3 antibody and protein A/G agarose beads. The precipitated DNA and input DNA were sequenced using an Illumina sequencer.  ChIP-Seq GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-09-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR563846 CRR563846_f1.fastq.gz
CRR563846_r2.fastq.gz
1,357.27
1,456.48
SubmitterWenbin Wang (sciencedb@gibh.ac.cn)
OrganizationNankai University
Date submitted2022-08-31