| Accession | CRX524180 |
| Organism | Ovis aries |
| Title | WT-2 |
| BioProject | PRJCA012608 |
| BioSample | SAMC954975 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The rRNA was removed from all samples with RINe > 8.0 and and no less than 2 μg. RNA purification, cDNA library construction and RNA sequencing were conducted by Berry Genomics company. Specifically, the NEBNext® UltraTM Directional RNA Library Prep Kit was used for Illumina® to make rRNA-free RNA sequencing libraries. Then, the firststrand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNaseH-), and cDNA second strand synthesis was performed using DNA polymerase I and RNase H. Each library was paired-end sequenced (2×150 bp) on an Illumina Novaseq6000 platform. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-10-31 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR589047 |
CRR589047_f1.fastq.gz
CRR589047_r2.fastq.gz
|
4,326.27
4,658.22
|
|
| Submitter | Mingming Chen (stdchan@qq.com) |
|---|
| Organization | China Agricultural University |
|---|
| Date submitted | 2022-10-20 |
|---|
