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Experiment information
Accession CRX562157
Organism Mus musculus
Title mouse_SSc_scRNAseq_day14_sample2
BioProject PRJCA013905
BioSample SAMC1020985
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Single cells were obtained as gel bead-in-emulsions (GEMs) using the 10X Genomics (Pleasanton, CA, USA) Chromium platform based on the manufacturer's protocol. Briefly, cell suspensions of skin tissue with reverse transcription master mix were loaded onto the 10X Genomics Single Cell 3' chip and partitioned into GEMs with gel beads precoated with oligonucleotides containing a poly-dT primer sequence to capture released mRNAs, a 16-bp 10X barcode for cell indexing, and a 10-bp unique molecular identifier (UMI) to distinguish between transcripts. After reverse transcription, barcoded cDNAs for each sample were amplified to generate a sufficient volume for library construction using the Single Cell 3' Reagent Kit (v3 chemistry). Libraries were subjected to 150-bp paired-end sequencing performed by Novogene (Beijing, China) on a NovaSeq 6000 system (Illumina, San Diego, CA, USA). ssRNA-Seq TRANSCRIPTOMIC SINGLE CELL unspecified PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-12-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR630035 CRR630035.tar.gz 2,798.77
CRR630036 CRR630036.tar.gz 10,890.66
CRR630037 CRR630037.tar.gz 2,865.38
CRR630038 CRR630038.tar.gz 39,784.02
CRR630039 CRR630039.tar.gz 16,026.02
CRR630040 CRR630040.tar.gz 16,359.76
SubmitterYongfei Hu (yongfei_hu_joe@163.com)
OrganizationSouthern Medical University
Date submitted2022-12-18