| 实验编号 | CRX572680 |
| 物种名称 | Mus musculus |
| 标题 | WT3 |
| 项目编号 | PRJCA014366 |
| 样本编号 | SAMC1041781 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). First strand cDNA was synthesized using random hexamer primer and RNase H. Second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I and RNase H. The library fragments were purified with QiaQuick PCR kits and elution with EB buffer, then terminal repair, A-tailing and adapter added were implemented. The aimed products were retrieved and PCR was performed, then the library was completed. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-01-11 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR640937 |
CRR640937_f1.fq.gz
CRR640937_r2.fq.gz
|
1,540.15
1,565.13
|
|
| 提交者 | LIU CHANGMEI (liuchm@ioz.ac.cn) |
|---|
| 所属单位 | Institute of Zoology, Chinese Academy of Sciences |
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| 提交日期 | 2023-01-11 |
|---|
