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Experiment information
Accession CRX708848
Organism Brachypodium
Title Bdi21_HiFi
BioProject PRJCA017149
BioSample SAMC1255397
Platform Pacbio Sequel II
Library
Library name Construction protocol Strategy Source Selection Layout
Fresh young leaf tissue was collected from Bdi21 plants. We constructed SMRTbell libraries using a method described in a previous study. Genomic DNA was isolated and sheared by Megaruptor, followed by damage repair, end repair, ligation with known adapters, enzyme digestion, size-selection, and generation of SMRTbell structure library. The qualified libraries were sequenced on the PacBio Sequel II platform (Pacific Biosciences) at BGI-ShenZhen Company, generating approximately 19.7 Gb of PacBio HiFi reads for the Bdi21 genome. WGS GENOMIC other SINGLE
Processing
Release date2024-02-09
Run
Run accession Run data file information
File nameFile size (MB)
CRR789935 CRR789935.bam 493,328.68
SubmitterHandong Su (shandong@mail.hzau.edu.cn)
OrganizationNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
Date submitted2023-05-24
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Experiments(4)