| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Fresh young leaf tissue was collected from Bst99 plants. We constructed SMRTbell libraries using a method described in a previous study. Genomic DNA was isolated and sheared by Megaruptor, followed by damage repair, end repair, ligation with known adapters, enzyme digestion, size-selection, and generation of SMRTbell structure library. The qualified libraries were sequenced on the PacBio Sequel II platform (Pacific Biosciences) at BGI-ShenZhen Company, generating approximately 19.4 Gb of PacBio HiFi reads for the Bst99 genome. |
WGS |
GENOMIC |
other |
SINGLE
|
|
