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实验基本信息
实验编号 CRX727873
物种名称 Mus musculus
标题 PBAT.P20-cKO.Rep1a
项目编号 PRJCA017857
样本编号 SAMC2819017
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
PBAT library preparation. Briefly, 5 ng genomic DNA together with 60 pg unmethylated lambda DNA (ThermoFisher) were subjected to bisulfite conversion followed by column-based purification using the EZ DNA methylation-direct kit (Zymo Research, D5021). Next, the random priming, library amplification and sequencing were consistent with the method used in the preparation of single-cell DNA library. Bisulfite-Seq GENOMIC RANDOM PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2023-07-09
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR810094 CRR810094_f1.fq.gz
CRR810094_r2.fq.gz
3,349.64
3,803.82
提交者Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn)
所属单位Southern Medical University
提交日期2023-06-25