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Experiment information
Accession CRX744187
Organism Mus musculus
Title RWT_5
BioProject PRJCA007579
BioSample SAMC2926425
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
The heart tissues were thoroughly ground at low temperatures. The tissue pellets were resuspended in 500 µl ice-cold lysis buffer with Protease Inhibitor Cocktail: 10 mM Tris·HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% NP-40. The tubes were incubated for 60 min with vortexing every 10 min to release nuclei. RNA-Seq TRANSCRIPTOMIC size fractionation PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2024-06-26
Run
Run accession Run data file information
File nameFile size (MB)
CRR827155 CRR827155_f1.fastq.gz
CRR827155_r2.fastq.gz
1,463.03
1,508.02
SubmitterFuchou Tang (tangfuchou@pku.edu.cn)
OrganizationPeking University
Date submitted2023-07-19