| Accession | CRX793854 |
| Organism | Oryza sativa |
| Title | STS-PCR-seq of sop10-2-3 |
| BioProject | PRJCA019796 |
| BioSample | SAMC3065314 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The above-ground parts from wild-type and sop10-2 seedlings at 12 DAP were used to isolate total RNA. Each RNA sample was extracted from six independent seedlings. Two biological replicates were used to analyze editing by STS-PCR-seq. To this end, the total RNA was reverse transcribed using random primers to generate cDNA. The 34 protein-coding mitochondrial transcripts were amplified from the cDNA, and the RT482 products were mixed in equimolar ratios. High-throughput sequencing and data analysis were performed according to Wang et al. (2019) PloS Genet 15:e1008305. |
OTHER |
OTHER |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-09-18 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR880137 |
CRR880137_f1.fq.gz
CRR880137_r2.fq.gz
|
204.47
211.85
|
|
| Submitter | Chunhui Xu (chunhuixu@sdu.edu.cn) |
|---|
| Organization | Shandong University |
|---|
| Date submitted | 2023-09-15 |
|---|
