| 实验编号 | CRX793857 |
| 物种名称 | Oryza sativa |
| 标题 | STS-PCR-seq of 9311-3 |
| 项目编号 | PRJCA019796 |
| 样本编号 | SAMC3065317 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The above-ground parts from wild-type and sop10-2 seedlings at 12 DAP were used to isolate total RNA. Each RNA sample was extracted from six independent seedlings. Two biological replicates were used to analyze editing by STS-PCR-seq. To this end, the total RNA was reverse transcribed using random primers to generate cDNA. The 34 protein-coding mitochondrial transcripts were amplified from the cDNA, and the RT482 products were mixed in equimolar ratios. High-throughput sequencing and data analysis were performed according to Wang et al. (2019) PloS Genet 15:e1008305. |
OTHER |
OTHER |
RT-PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2023-09-18 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR880140 |
CRR880140_f1.fq.gz
CRR880140_r2.fq.gz
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223.49
241.18
|
|
| 提交者 | Chunhui Xu (chunhuixu@sdu.edu.cn) |
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| 所属单位 | Shandong University |
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| 提交日期 | 2023-09-15 |
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