| Accession | CRX795088 |
| Organism | human metagenome |
| Title | MEF27 |
| BioProject | PRJCA019468 |
| BioSample | SAMC3068794 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Sequencing libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350 bp, and then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Finally, PCR products were purified (AMPure XP system), and libraries were analyzed for size distribution by an Agilent 2126 Bioanalyzer and quantified using real-time PCR. |
OTHER |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-09-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR881447 |
CRR881447_f1.fq.gz
CRR881447_r2.fq.gz
|
1,918.33
2,098.98
|
|
| Submitter | Yue Fan (chromefan@126.com) |
|---|
| Organization | Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College |
|---|
| Date submitted | 2023-09-17 |
|---|
