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Experiment information
Accession CRX795100
Organism human metagenome
Title MEF39
BioProject PRJCA019468
BioSample SAMC3068806
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Sequencing libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350 bp, and then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Finally, PCR products were purified (AMPure XP system), and libraries were analyzed for size distribution by an Agilent 2138 Bioanalyzer and quantified using real-time PCR. OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-09-21
Run
Run accession Run data file information
File nameFile size (MB)
CRR881459 CRR881459_f1.fq.gz
CRR881459_r2.fq.gz
1,462.07
1,519.47
SubmitterYue Fan (chromefan@126.com)
OrganizationPeking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College
Date submitted2023-09-17