| 实验编号 | CRX795103 |
| 物种名称 | human metagenome |
| 标题 | MEF42 |
| 项目编号 | PRJCA019468 |
| 样本编号 | SAMC3068809 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Sequencing libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350 bp, and then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Finally, PCR products were purified (AMPure XP system), and libraries were analyzed for size distribution by an Agilent 2141 Bioanalyzer and quantified using real-time PCR. |
OTHER |
GENOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2023-09-21 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR881462 |
CRR881462_f1.fq.gz
CRR881462_r2.fq.gz
|
1,678.97
1,783.66
|
|
| 提交者 | Yue Fan (chromefan@126.com) |
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| 所属单位 | Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College |
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| 提交日期 | 2023-09-17 |
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