| Accession | CRX824339 |
| Organism | Mus musculus |
| Title | Ribo_KO1 |
| BioProject | PRJCA020241 |
| BioSample | SAMC3092948 |
| Platform | Illumina Nextseq 500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Cells were treated with 100 μg/mL cycloheximide, lysed and centrifuged to collect the supernatant, which was then nuclease digested with RNase I and DNase I to obtain the ribosome-protected fragments (RPFs). RPFs were separated by the RNA Clean and Concentrator-27 kit and purified by magnet beads. The library was constructed using NEBNext® Multiple Small RNA Library Prep Set for Illumina |
Ribo-seq |
TRANSCRIPTOMIC |
other |
SINGLE
|
|
| Processing |
Planned read length (bp): 75
|
| Release date | 2024-11-13 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR911173 |
CRR911173.fq.gz
|
1,377.78
|
|
| Submitter | Quan Yuan (yuanquan@scu.edu.cn) |
|---|
| Organization | West China Hospital of Stomatology, Sichuan University |
|---|
| Date submitted | 2023-10-06 |
|---|
