| 实验编号 | CRX867417 |
| 物种名称 | Mus musculus |
| 标题 | H3K27ac CUT&Tag-seq of T1-E14.5, replicate 3 |
| 项目编号 | PRJCA021396 |
| 样本编号 | SAMC3180210 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Nuclei were extracted from the sample after homogenization, and bound to Concanavalin A-coated magnetic beads, followed by the incubation with the Abcam H3K27ac Rabbit pAb. Nuclei were then successively incubated with the ABclonal Goat anti-Rabbit IgG H&L, pA-Tn5 adapter complex preparation, and tagmentation buffer, followed by Proteinase K treatment to stop tagmentation. DNA was extracted using the Beckman Coulter AMPure XP beads, and purified using the AMPure XP beads after the amplification with the NEBNext HiFi 2× PCR Master Mix, universal i5 primer and uniquely barcoded i7 primer. Library was assessed by the Life Invitrogen Qubit 3.0 and the Agilent Bioanalyzer 2100 system. |
OTHER |
GENOMIC |
other |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2023-11-24 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR956291 |
CRR956291_f1.fq.gz
CRR956291_r2.fq.gz
|
2,081.87
2,108.89
|
|
| 提交者 | Wandong Bao (baowandong@mail.kiz.ac.cn) |
|---|
| 所属单位 | Kunming Institute of Zoology, Chinese Academy of Sciences |
|---|
| 提交日期 | 2023-11-24 |
|---|
