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All isolates were cultivated in ryptic soytone broth medium (HuanKai Microbial, China) overnight at 37 °C. After genomic DNA was extracted using the Bacteria Genomic DNA Extraction kit (Magen Biotech, China). DNA integrity and quantity were assessed on a Nanodrop UV-vis spectrophotometer (Thermo Fisher, USA) and 1% agarose gel electrophoresis visualized with ethidium bromide in a ChemiDoc XRS photodocumenter (BioRad, USA). Paired-end libraries were constructed using the TruSeq DNA Sample Prep Kit (Illumina, USA) according to the manufacturer’s instructions. The libraries were subjected to purification using AMPure XP Beads (Beckman Coulter, USA). For the sequencing phase, the Illumina X-10 platform was employed, generating 2 × 150 bp paired-end reads, consequently accumulating a total of 2 GB data per individual sample. |
WGS |
GENOMIC |
RANDOM |
PAIRED
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