| Accession | CRX1112830 |
| Organism | Mus musculus |
| Title | yolk sac ECs |
| BioProject | PRJCA026998 |
| BioSample | SAMC3877651 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
For 10X Genomics data, single-cell suspensions were loaded on a Chromium Single Cell Controller (10X Genomics) to generate single-cell gel beads in emulsion (GEMs) by using Single Cell 30 Library and Gel Bead Kit V2 (10X Genomics, 120237). RNAs barcoding were obtained through reverse transcription in individual GEMs. Barcoded cDNAs were pooled and cleaned up using beads (Invitrogen, 37002D). Single-cell RNA-sequencing (scRNA-seq) libraries were prepared using Single Cell 30 Library Gel Bead Kit V2 (10X Genomics, 120237) following the manufacture’s introduction. Sequencing was performed on an Illumina HiSeq X Ten with pair end 150 bp (PE150). |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-08-31 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1207557 |
CRR1207557_f1.fq.gz
CRR1207557_r2.fq.gz
|
4,214.5
3,890.44
|
|
| Submitter | yongjie Li (liyongjie23@mails.ucas.ac.cn) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2024-06-22 |
|---|
