| Accession | CRX1117445 |
| Organism | Mus musculus |
| Title | induced pluripotent stem cells |
| BioProject | PRJCA027535 |
| BioSample | SAMC3957497 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted using TRIZOL Reagent (Life technologies) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100.Qualified total RNA was further purified by RNeasy micro kit and RNase-Free DNase Set.Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
RNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-07-09 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1212521 |
CRR1212521_f1.fastq.gz
CRR1212521_r2.fastq.gz
|
2,061.76
2,194.31
|
|
| Submitter | Ping Gao (pgao2@ustc.edu.cn) |
|---|
| Organization | South China University of Technology |
|---|
| Date submitted | 2024-07-01 |
|---|
