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The qualified DNA samples were randomly fragmented using a Covaris ultrasonic disruptor,and the entire library was prepared through steps such as end repair, addition of A-tails,addition of sequencing adapters, purification, and PCR amplification. Perform Agilent 2100 Bioanalyzer and qPCR testing on the library,and proceed to the next step of machine sequencing after passing the tests. After passing the library quality inspection,Different libraries are pooled according to their effective concentrations and target data volume requirements for Illumina sequencing. |
Hi-C |
GENOMIC |
DNAse |
PAIRED
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