| 实验编号 | CRX1215337 |
| 物种名称 | Rattus norvegicus |
| 标题 | CUT&Tag-seq for HDAC1 using Adv-Ctrl infected NRVMs, replicate 1 |
| 项目编号 | PRJCA030749 |
| 样本编号 | SAMC4234163 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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CUT&Tag assay was performed using NovoNGS CUT&Tag 3.0 High-Sensitivity Kit. Isolated NRVMs were enriched with ConA beads and incubated with primary antibody buffer of anti-HDAC1 antibody. The cells were then incubated with protein A/G-Tn5 transposome and the tagmentation buffer. The DNA fragments were extracted by Tagment DNA extract beads and the libraries were amplified using 5x AmpliMix. The DNA libraries were extracted by DNA clean beads for sequencing and paired-end 150-base reads were generated using the Illumina NovaSeq 6000 platform. |
OTHER |
GENOMIC |
PCR |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2024-11-01 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1329034 |
CRR1329034_r1.fq.gz
CRR1329034_r2.fq.gz
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1,247.17
1,239.83
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| 提交者 | chengqi lin (cqlin@seu.edu.cn) |
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| 所属单位 | Southeast University |
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| 提交日期 | 2024-10-11 |
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