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实验基本信息
实验编号 CRX1223054
物种名称 Mus musculus
标题 H3K27ac ChIPseq for E7.5 AM rep1
项目编号 PRJCA030749
样本编号 SAMC4251874
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
We performed the ChIP assay for histone modifications with specific antibodies against H3K4me3 (Abcam, 8580), H3K4me1 (Abcam, 8895), H3K27ac (Active Motif, 39133), and H3K9ac (Millipore, 06-942). ChIP libraries were prepared using NEBNext Ultra DNA library preparation kit (NEB, E7370) according to the manufacturer’s instructions. ChIP-Seq GENOMIC ChIP PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
Nominal size (bp): 120
发布日期2024-10-17
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1336783 CRR1336783_r1.fastq.gz
CRR1336783_r2.fastq.gz
3,440.07
3,434.61
提交者chengqi lin (cqlin@seu.edu.cn)
所属单位Southeast University
提交日期2024-10-14