| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The total RNA for each sample was extracted with the miRNeasy Mini Kit (Qiagen). Then the libraries were prepared following the standard protocols with Optimal Dual-mode mRNA library Prep Kit (BGI-Shenzhen, China) for strand-specific mRNA sequencing and MGIEasy Small RNA Library Prep Kit (BGI-Shenzhen, China) for small RNA sequencing, with two to four biological replicates for each samples. The mRNA libraries passing the quality control were processed on T7 platform (BGI-Shenzhen, China), generating paired-end 150 bp reads for mRNA. For small RNA libraries, except for those from the samples of ein5 drb4 and ein5 drb4 dcl2, singled-end 50 bp reads were generated on DNBSEQ platform (BGI-Shenzhen, China). The sequencing of small RNA libraries constructed from ein5 drb4 and ein5 drb4 dcl2 were conducted on Hiseq X Ten platform (BGI-Shenzhen, China). |
ncRNA-Seq |
OTHER |
size fractionation |
SINGLE
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