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The samples were then thawed and ground three times. After centrifugation (10 min, 15,000 g), the supernatant was collected. This supernatant was filtered through a 0.45 mm filter (Millipore) to remove particles the size of eukaryotic and bacterial cells. Subsequently, 200 uL of supernatant from each pool was treated with a nuclease mixture to reduce the concentration of free (non-viral-encapsulated) nucleic acids. The remaining total nucleic acids were isolated using the QIAamp MinElute Virus Spin Kit according to the manufacturer's protocol. Seventy libraries were constructed using the Nextera XT DNA Sample Preparation Kit (Illumina), and 250 base pair sequencing was performed on each library using the MiSeq Illumina platform. |
WGS |
METAGENOMIC |
RANDOM PCR |
PAIRED
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