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实验基本信息
实验编号 CRX1472974
物种名称 Danio rerio
标题 RNA-Seq of danio rerio: adult testis vehicle 2h Replicate 3
项目编号 PRJCA035434
样本编号 SAMC4601737
测序平台 Illumina HiSeq X Ten
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Samples in triplicates were collected 2 hr after vehicle treatment i.p., each sample from three individuals. A total amount of 3 g RNA per sample was used for the RNA sample preparations. Sequencing libraries were generated using NEBNextUltra RNA Library Prep Kit (NEB, USA) following the manufacturers instructions. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumia) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2025-04-03
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1593610 CRR1593610_r1.fq.gz
CRR1593610_r2.fq.gz
1,502.33
1,520.18
提交者Taole LIU (liutaole@suda.edu.cn)
所属单位Soochow University
提交日期2025-01-22