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High-quality genomic DNA was extracted and subjected to purity, concentration, and integrity assessments using Nanodrop, Qubit, and 0.35% agarose gel electrophoresis. Large DNA fragments were recovered using the BluePippin automated nucleic acid recovery system. Library preparation was performed using the SQK-LSK109 ligation kit, involving DNA damage repair and end repair, followed by magnetic bead purification. Adapter ligation was then carried out, followed by another round of magnetic bead purification. The library was quantified using Qubit and subsequently subjected to sequencing. |
WGS |
GENOMIC |
RANDOM |
SINGLE
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