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Canis lupus familiaris

Basic Information

Canis lupus familiaris
Accession: ICG00131
Latin Name: Canis lupus familiaris
Common Name: Domestic dog
Taxonomy ID: 9615
Description: Canis lupus familiaris, as a member of genus Canis (canines) that forms part of the wolf-like canids, is the most widely abundant carnivore. Pet domestic dogs are highly responsive to human actions and attentional states.

Gene Summary

mRNA

ICG00131-5
HMBS RPL13A GAPDH
ICG00131-4
RPL8
ICG00131-3
B2M HMBS RPL13A SDHA TBP YWHAZ
ICG00131-2
RPS5 RPL8 RPS19 RPL32 GAPDH MRPS7 RPL13A
ICG00131-1
RPL13A RPL32

Studies (5)

ICG00131-1

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPL13A Ribosomal Protein L13
Bone Marrow
Colon
Duodenum
Heart
Kidney
Liver
Lung
Lymph Node
Skeletal Muscle
Pancreas
Spleen
Stomach
 AJ388525
F: GCCGGAAGGTTGTAGTCGT
R: GGAGGAAGGCCAGGTAATTC
 87 85 SYBR
RPL32 Ribosomal Protein L32
Bone Marrow
Colon
Duodenum
Heart
Kidney
Liver
Lung
Lymph Node
Skeletal Muscle
Pancreas
Spleen
Stomach
 XM_848016.1
F: TGGTTACAGGAGCAACAAGAAA
R: GCACATCAGCAGCACTTCA
 100 87 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

Contact

Name: Iain R. Peters
Email: I.R.Peters@Bristol.ac.uk
Institution: School of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK

Reference

Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies.
Peters IR, Peeters D, Helps CR, Day MJ.
Veterinary immunology and immunopathology. 2007-01-31

ICG00131-2

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPS5 Ribosomal Protein S5
Whole Brain
Forebrain
Cerebrum
 XM_533568
F: GGATGACCGAGTGGGAGA
R: TGCAATGTAGTCCTGCAAAGA
 122 60 SYBR
RPL8 Ribosomal Protein L8
Whole Brain
Forebrain
Cerebrum
 XM_532360
F: AGGTCATTTCTTCCGCCAA
R: AGGATGCTCCACAGGATTCA
 164 60 SYBR
RPS19 Ribosomal Protein S18
Forebrain
Cerebrum
Diencephalon
Hindbrain
 XM_533657
F: GTGAACCAGCAGGAGTTCGT
R: GGAGGTACAGGTGCCGTG
 175 60 SYBR
RPL32 Ribosomal Protein L32
Diencephalon
 XM_844472
F: ATCGCTGGTTGGCTCAGTAG
R: AGAGAGAGGCAGAGACGGTG
 112 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Hindbrain
 NM_001003142
F: CACAGTCAAGGCTGAGAACG
R: CACCAGCATCACCCCATTT
 101 60 SYBR
MRPS7 Mitochondrial Ribosomal Protein S7
Metencephalon
 XM_846915
F: GATCCCTCATGATGCAGACTC
R: GGCACAGGGACCTGATAAAA
 196 60 SYBR
RPL13A Ribosomal Protein L13
Metencephalon
 AJ388525
F: AGGTTGTAGTCGTGCGCTGT
R: CATGCCTCTAACTGTCCGC
 167 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Kyu-Tae Chang
Email: changkt@kribb.re.kr
Institution: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk 363-883, Republic of Korea

Reference

Selection of internal reference genes for normalization of quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis in the canine brain and other organs.
Park SJ, Huh JW, Kim YH, Lee SR, Kim SH, Kim SU, Kim HS, Kim MK, Chang KT.
Molecular biotechnology. 2013-05-01

ICG00131-3

Articular Connective Model
Tissues
Cell Types
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
B2M Microglobulin
Ruptured Cranial Cruciate Ligament
 XM_535458
F: CCTTGCTCCTCATCCTCCT
R: TGGGTGTCGTGAGTACACTTG
 83 60 SYBR
HMBS Hydroxymethylbilane Synthase
Lps Stimulated Cell Lines
 XM_546491
F: GCATGTATGCTGTGGGTCAG
R: GCATGTCCTGGTCCTTGG
 63 60 SYBR
RPL13A Ribosomal Protein L13
Infrapatella Fat Pad
Osteoarthritic Cartilage
 ENSCAFG00000003633
F: CTGCCCCACAAGACCAAG
R: GGGATCCCATCAAACACCT
 65 60 SYBR
SDHA Succinate Dehydrogenase Complex
Osteoarthritic Cartilage
Canine K6 Cell Line
Canine Dh82 Cell Line
 XM_535807
F: GGTGGCACTTCTACGACACC
R: ATGTAGTGGATGGCGTCCTG
 67 60 SYBR
TBP TATA-binding Protein
Ruptured Cranial Cruciate Ligament
 ENSCAFG00000004119
F: TCCACAGCCTATCCAGAACA
R: CTGCTGCTGTTGTCTCTGCT
 67 60 SYBR
YWHAZ Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta Polypeptide
Infrapatella Fat Pad
Canine K6 Cell Line
Canine Dh82 Cell Line
 XM_532287
F: GTTACTTGGCCGAAGTTGCT
R: TGCTTGTTGTGACTGATCCAC
 65 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

Contact

Name: Philip JR Day
Email: Philip.J.Day@manchester.ac.uk
Institution: Centre for Integrated Genomic Medical Research, The Manchester Interdisciplinary Biocentre, University of Manchester, M1 7ND, UK

Reference

Expression stability of commonly used reference genes in canine articular connective tissues.
Ayers D, Clements DN, Salway F, Day PJ.
BMC veterinary research. 2007-05-07

ICG00131-4

Internal Disease
Hematological Disease
Tissues
Non-Cancer Disease Studies

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPL8 Ribosomal Protein L8
Healthy Control Dogs
Surgery Within The Pre- Ceding 24 Hours
Dogs With Miscellaneous Internal Diseases
Hematologic Disease
Systemic Inflammatory Disease
Immunemediated Hemolysis
 XM_532360
F: CCATGAATCCTGTGGAGC
R: GTAGAGGGTTTGCCGATG
 64 58 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Christine J Piek
Email: C.J.Piek@uu.nl
Institution: Department of Clinical Sciences of Companion Animals, Utrecht, Utrecht University, PO Box 80154, 3508 TD Utrecht, The Netherlands

Reference

Leukocyte count affects expression of reference genes in canine whole blood samples.
Piek CJ, Brinkhof B, Rothuizen J, Dekker A, Penning LC.
BMC research notes. 2011-02-09

ICG00131-5

Brain Regions
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
HMBS Hydroxymethylbilane Synthase
Frontal Lobe
Parietal Lobe
Occipital Lobe
Cerebellum
 XM_546491
F: TCACCATCGGAGCCATCT
R: GTTCCCACCACGCTCTTCT
 112 57 SYBR
RPL13A Ribosomal Protein L13
Temporal Lobe
Hippocampus
 AJ388525
F: GCCGGAAGGTTGTAGTCGT
R: GGAGGAAGGCCAGGTAATTC
 87 57 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Thalamus
 NM_001003142
F: TGTCCCCACCCCCAATGTATC
R: CTCCGATGCCTGCTTCACTACCTT
 100 57 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Quirine E. M. Stassen
Email: Q.E.M.Stassen@uu.nl
Institution: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, PO Box 80154, 3508 TD Utrecht, The Netherlands

Reference

Reference genes for reverse transcription quantitative PCR in canine brain tissue.
Stassen QE, Riemers FM, Reijmerink H, Leegwater PA, Penning LC.
BMC research notes. 2015-12-09