GHET1

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GHET1, which is located in an intergenic region on chromatin 7, acting as a prognostic factor for gastric carcinoma that promotes c-Myc mRNA stability and expression and the growth of gastric carcinoma functions as an oncogene in gastric carcinoma.

Annotated Information

Name

GHET1 promotes gastric carcinoma cell proliferation [1].

GHET1: gastric carcinoma proliferation enhancing transcript 1 (HGNC nomenclature)

"gastric carcinoma high expressed transcript 1", lncRNA-GHET1 [1]

Characteristics

GHET1, gastric carcinoma proliferation enhancing transcript 1, located in an intergenic region on chromatin 7[1].

Function

GHET1 increases c-Myc expression and mRNA stability [1].

GHET1 is up-regulated in gastric carcinoma tissues in comparison with adjacent normal gastric tissues, and GHET1 up-regulation is correlated with tumor size and invasion. High GHET1 expression in gastric carcinoma tissues indicates poor overall survival of gastric carcinoma patients. Enhanced GHET1 expression promotes proliferation of gastric carcinoma cells in vitro and in vivo, while inhibition of GHET1 expression inhibits gastric carcinoma cell proliferation , which indicates that GHET1 functions as an oncogene in gastric carcinoma[1].

GHET1 associates with IGF2BP1 and enhances the physical interaction between c-Myc mRNA and IGF2BP1 protein[1].

Diseases

  • gastric carcinoma[1]

Expression

Expression of GHET1 in clinical samples and its association with patient's prognosis[1]

GHET1 expression levels were significantly increased in gastric carcinoma tissues compared with adjacent normal gastric tissues[1].

siRNAs specifically targeting GHET1 were synthesized by Invitrogen, siRNAs specifically targeting c-Myc were synthesized by GenePharma (Shanghai, China), and siRNAs specifically targeting IGF2B1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA)[1].

siRNA Sequence
c-Myc 5'-GGUGAUCCAGACUCUGACCUU-3'[1]
GHET1 5'-CGGCAGGCATTAGAGATGAACAGCA-3'[1]

For each sample, gene expression was normalized to 18S rRNA. The primer sequences used were as follows:

Primer Forward primer Reverse primer
GHET1 5'-CCCCACAAATGAAGACACT-3' 5'-TTCCCAACACCCTATAAGAT-3'[1]
GAPDH 5'-GGTCTCCTCTGACTTCAACA-3' 5'-GTGAGGGTCTCTCTCTTCCT-3'[1]
c-MycGCTATGGGCAAAGTTTCGTG 5'-GGGCTTTATCTAACTCGCTGTA-3' 5'-GCTATGGGCAAAGTTTCGTG'[1]
18S rRNA 5'-ACACGGACAGGATTGACAGA-3' 5'-GGACATCTAAGGGCATCACA-3'[1]

Sequence

>gi|732170727|ref|NR_130107.1| Homo sapiens gastric carcinoma proliferation enhancing transcript 1 (GHET1), long non-coding RNA

000001 AGCCACCGCA AGGTACCAGA GAGCCGCCCA ATCTGCGCTG GCCTCCTTTA TCTGTTTGCT GTGTGACCCT CCGGACAGCT 000080
000081 CTGTGGGACC TGGAGGAAAC TGTGAGCCCT ACAGATAAAG TCTGTGCTTC CAGCGTCGGT AAATCCCAGC AAGCGCCACT 000160
000161 ACCTGGAGGC TGACACTTTC CCATCTTGGC AGTCAGCTCC CTACAGAGGT GCTTCTCCCA CTGCCCAAGA TCCCTGCCTC 000240
000241 CTCCTTAAAC CCCAACTGAT AGAACAGAAA CCACCACCTA AGGAACGTGA AGGGGAAGCA TCTGAAACAC CAGAAGATCT 000320
000321 TGGATTGTTC CTGAGGACTT CAGAATCTAC AGGGACAAAA GGCAGAAGCA GGAAGTCCGA AGTAAAGATG ACTAAGAAAA 000400
000401 GGCGGGTGGA AAAGATTTCT GTGAAAGTGT CAGAGGCAAA GCTCTGAAAG ACCTGAAACT CATGCCGAAA GCAAAGAACA 000480
000481 GCAAAAAGAC AGTAGGGATG TGTCTGGAGC AACAAGGATC GGGCTGTTCC TGGGGCAGCC GAATTCACAG CTCTGTTTTA 000560
000561 CTCCTAATGG AGGATCTTCC TGCTGCAGCG GCGAGTAGAC CTTACTGTAA AGGTGCAGGC AAGGGGAGGG GAAGGGCAGG 000640
000641 CAGCTGCCCA TTGGAGTTCA CATCTCCAAG CCTGAGAAAA TGTGTTCCTG GATGCCCCAA GCCAATGGAA AAGCAGAATC 000720
000721 ATTGACTATG TTTGACTCCT TCTGGTACCT AGCACAGTAT CTGGGACACA GCAGGTGCTA TGTAAATGTG TTGAGTGAAT 000800
000801 GAATGAATGA ATGCCATTGA GGAGAATGAA TAACCCCAGA AAACTAGACA TGGGTAGATG TTGTCCTAAT TTCAAAAGAG 000880
000881 GGGAAAATGA GAGTGAGAGT TTCATAAATG ACAATCTCTA AACGATGTTG CTCTCTAGCA AAATTCTAGA ATGGATTATT 000960
000961 TTAAGAACAA AGCAGGTAAA CATTGGCAGC CAACTTGAAA TTAATAAGAA AAAGGTGTGC GCCAGAATAG TTTTATTTCC 001040
001041 TTTTGTTTTT CATTGTACAG CTTGATTTGA CTAAGTTGTT TATACCTTTC ACTCTGCCTT TGCATGCACT GGGGGTGATC 001120
001121 TAGTCTACTA TGTGGACTGG AAGTCCACCC AGTGGCCAGG CTCAGAGGCA GGGAAGGGGG GTTGGTGCCA TTCTGGGAGA 001200
001201 GGAGGTTTCT GGGGCTGTTT CTCAGCCAGA TCCTGTCTCA TCTGTTTCCA ACAACCCCAC AAATGAAGAC ACTGAAGCTG 001280
001281 ACTAATCAAA TTGACTGATG CCCCAATAGG TGTCATGATA TGCAGTGAGA ATCCGGCAGG CATTAGAGAT GAACAGCACG 001360
001361 GGCTGCAGCC ACATTATCTC AGCTTGAGTC CTAGTTTTCA CCAGCTACTA GCTAGCTATC TCTGGTCAAG TCACTTACCT 001440
001441 TGCTTGAGCC TCAGTTTCTC CATCTAGAAA ATGGTCATAA TCGAAGTATC TATCTTATAG GGTGTTGGGA AGATTAACTG 001520
001521 AGAAGCCACA TAGAGTTTAC TGGCACATTA AAAAAAGAAA CTATAAGAAT GTTAGCTGTC CTTTTGCACG GCAAAAGGAA 001600
001601 CAGTAAGCAG AGTAAACAGA CAACCCACAG AGTGGGAGAA AATCTTCCCA ATCTATACAT CCGACAAAGG ACTAACATCC 001680
001681 AGAATCTACG AGGAACTCAA ATTAGCAAGA AAAAAACAAT CCCATCAAAA ACTGGCCTAA GGACATGAAT AGACAATTCT 001760
001761 CAAAAGAAGA TATACAAATG GCCAACAAAC ATATGAAAAA GTGCTCAATA TCACTAATGA TCAGGGAAAT GCAAATCAAA 001840
001841 ACCACAATGC AATACCACAT TACTCCTGCA AGAATGGCCA TAATAAAAAA AAAATCAAAA AAAAAAAAAA AAA

Labs working on this lncRNA

  • Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China [1].

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Yang F, Xue X, Zheng L, Bi J, Zhou Y, Zhi K, et al. Long non-coding RNA GHET1 promotes gastric carcinoma cell proliferation by increasing c-Myc mRNA stability[J]. The FEBS journal. 2014,281(3):802-13.
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