HOXB-AS1
Mouse counterpart of lncRNA HOXB-AS1, Hoxb3os results in hyperactivation of mTORC1 and enhanced mitochondrial respiration.
Contents
Annotated Information
Name
HOXB-AS1: HOXB cluster antisense RNA 1 (HGNC nomenclature). Mouse orthologue: Hoxb3os [1].
Characteristics
HOXB-AS1 comprises of four transcripts that partially overlap with HOXB2 and HOXB3 [1].
Function
Autosomal dominant polycystic kidney disease (ADPKD) is primarily caused by mutations in two genes, PKD1 and PKD2 lncRNAs, defined by a length >200 nucleotides and absence of a long ORF, have recently emerged as epigenetic regulators of development and disease.HOXB-AS1 is a novel lncRNA that is down-regulated in ADPKD and regulates mTOR signaling and mitochondrial respiration. Kidney-specific, evolutionarily conserved lncRNA called Hoxb3os is down-regulated in cystic kidneys from Pkd1 and Pkd2 mutant mice. The human ortholog HOXB3-AS1 is down-regulated in cystic kidneys from ADPKD patients. Hoxb3os, the mouse ortholog, is highly expressed in renal tubules in adult wild type mice, whereas its expression is lost in the cyst epithelium of mutant mice [1].
Regulation
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Diseases
- Autosomal dominant polycystic kidney disease (ADPKD) [1]
Expression
Expression analysis utilizing primer sets that detected multiple HOXB-AS1 isoforms reveals that HOXB-AS1 is downregulated by 60% in cystic kidneys from humans with ADPKD [1].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-AGAGCTGGAACTAGAAACT- 3' | 5'-AACCTAGCTTATTACTGCTTC- 3'[1] |
Labs working on this lncRNA
- Department of Medicine, University of Minnesota, Minneapolis, Minnesota, 55455. [1]
- McDermott Center for Human Growth and Development, University of Minnesota, Minneapolis, Minnesota, 55455. [1]
- Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, 55455. [1]
- Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390. [1]
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Aboudehen K, Farahani S, Kanchwala M, Chan SC, Avdulov S, Mickelson A, Lee D, Gearhart MD, Patel V, Xing C, Igarashi P. Long noncoding RNA Hoxb3os is dysregulated in autosomal dominant polycystic kidney disease and regulates mTOR signaling. Journal of Biological Chemistry. 2018.
Sequence
>gi|100874362|ref|NR_102279.1| Homo sapiens HOXB cluster antisense RNA 1 (HOXB3-AS1), long non-coding RNA
000081 GGGAATTCGT GGTCACGGGA CCGGCCTCCG CCAATCGCTC GTCTGGGCCT GGTGGAAAAC AGAGAGCATT ATTTGGTTTA 000160
000161 ATTGATTCAA AAACACCAGA GGACCACGAC CAGGACACCA TGAGCCCCCA TTCCTACACC AGTGCCTCAC ATGCTCTCTC 000240
000241 CCCAATGCCT CCGTTTCTCC AGAAAAGCTA GAGGAACCTT TTCCTCATCC CCTTCGAAGG AAAAGATGAG TGAGGATCTC 000320
000321 CGCGTTGGAG GAAGAGGGAG TCGAAAGAGG CTTCCACTCT TTGAAAAAGA CCCAATGGTG GTTGGGCTTC GGGTCACGTG 000400
000401 ACACCTTCTT TGTGGCTCCG GAAGAGGATT GTGAAGTTTA GGTTTCGTGG AAGAAACCGA TAATTGAGGA AAAAAAAAAA 000480
000481 TCAAGATTTG GGGGGAATTA CCTACAAAGA TGTAAGGTGT GTTAATTTGG GCGCTTCTTG GATTCTACCC TGAGGAGGAG 000560
000561 GCGCGTGGTG AGAGGAGAGC TCCACCAGCT GCGCGCTCGT GTACGCCGTC CGCGCCCGCT TGGACGCCGC CGACCCCGGG 000640
000641 GGGCTCTTGT CCCCTCCCCC GCCGCCGCCG CCACCGCCCC CGCTGCCACC ACTGCCTCCG CCGCCGCCGC CACCGCCGCC 000720
000721 GCCACCACAG CCCTCTGCTG GATCCGAGGG GGAGGGGTGG GAAGGGGAGA AAATGAAATA AAAGATAATT ACTGAAC
