RAB11B-AS1
RAB11B antisense RNA (RAB11B-AS1) is a long non-coding RNA which is markedly down-regulated in human osteosarcoma (OS) and associated with OS metastasis and poor prognosis.
Contents
Annotated Information
Name
RAB11B-AS1: RAB11B antisense RNA 1 (HGNC nomenclature).
Characteristics
RAB11B-AS1 locates at chromosome 19q13.2 and belongs to Ras family. RAB gene is well known for its role in tumor activation and progression [1].
Function
RAB11B-AS1 significantly facilitates proliferation, migration and invasiveness and prevents apoptosis of OS cells and results in lower sensitivity to cisplatin in these cells. In contrast, up-regulation of RAB11B-AS1 suppresses the aggressive behaviors of OS cells [1]. RAB11B-AS1 prevents osteosarcoma development and progression via inhibiting RAB11B expression, indicating RAB11B-AS1 as a potential therapeutic target for osteosarcoma [1].
Regulation
Majority of RAB11B-AS1 is shown to be localized in nucleus other than in cytoplasm of cells, suggesting the lncRNA as a transcriptional regulator in osteosarcoma cells [1].
Diseases
- Osteosarcoma (OS) [1]
Expression
Down-regulation of RAB11B-AS1 elevates its sense-cognate gene RAB11B expression at both mRNA and protein levels. RAB11B-AS1 expression correlates negatively with RAB11B expression in OS tissues [1].
| Experiment | Forward primer | Reverse primer |
|---|---|---|
| qRT-PCR | 5'-GGAACATGTTTACATGGACTTTGT- 3' | 5'-TCTTTGTTCTTGTTTGTTTTCTTTCT- 3' [1] |
Labs working on this lncRNA
- Academy of Orthopedics, Guangdong Province, Department of Orthopedics, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, China. [1]
- The State Key Lab of Respiratory Disease, The Institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity, Guangzhou Medical University, Guangzhou 510182, China. [1]
References
Sequence
>gi|100507567|ref|NR_038237.1| Homo sapiens RAB11B antisense RNA 1 (RAB11B-AS1), long non-coding RNA
000081 CTGCCCGGTC GGCTCCGGCC TTCCCGGCTG CCTGACCCGC CGGGCCCCAA GCCCCGGGCT CCGGCCTGGG CCCAGCGGCC 000160
000161 TGGAGCGCGG CCTTGGCCCG TCTGCCCGCC GCCTCGCCGC CACGACTTCG CCCGTCTGTG CCCCACCGGC CGCACCTTTG 000240
000241 AATAGGTAGT CGTACTCGTC GTCCCGGGTC CCCATTGTCC TGGCGCTTCC GGCGGGATCG GCGACTCCGC AGCCCCACCA 000320
000321 CAAACACCCG ACGGGGGCGG AGCCGGCGCC GCGCAGAGCG GCGGTTGAAT GGCCTATCAG CGGCGAGTGG AGTAGCGACG 000400
000401 GGCACCCAGC GAAGCCAATC AGAGATGGAA GTAGTGCTCT GAGGGTGGGC GCCGCTTGGT ACCACCCTCC TCGCCCTCGG 000480
000481 TGTCCTGGAG AAAGGCGGAA GGAATGCGGA CCTTTTTGAA GTGACGGACG CGCCAGCCTA TCAGGGGCGA GCTCAAGAGG 000560
000561 GCGGGGCGGA AGACTGCAGG AATGAAATGG ATTGACAGAC CAAATAACTA ATGAGAGGCT TGATTGAGAA CCTACCCGAC 000640
000641 TATCAGAGGA CCTGTCCGGG AAGAGAAATG GGGCTACGTC CAGACAGAAT CTCGCTCTGT TGCCTGGGCT GGAGTGCAGT 000720
000721 GGCACTGTAT CCGGAATTGG GGGGTTCTTG GTCTCACTGA CTTCAAGAAT GAAGCCATGG ACCCTTGCGA GATGGGGGTT 000800
000801 CCACCATGTT GGCCCGGCTG GTCTCGAACT GCTGACCTCA AGTGACCTGC CTGTCTCGGC CTCACAAATG GCTGGGATTA 000880
000881 CAGGCACGAG ACACCGTGCC TGGCCCTGGG AACATGTTTA CATGGACTTT GTTCACTTTT TAAAACAAAC TGAGGGTAAC 000960
000961 TTACTAGATC TGTGAACTAA AGAAAGAGAA AAAAAAGAAA GAAAACAAAC AAGAACAAAG AAAAAAAAAA AAAA
