View source for Lnc-OR10H5-1:1

''UCA1''  Urothelial cancer associated 1 is a long noncoding RNA that is involved in the growth and tumorigenesis of various kinds of human cancer.

==Annotated Information==
===Name===
Approved symbol: UCA1

Approved name: urothelial cancer associated 1

HGNC ID: HGNC:37126

Previous names: urothelial cancer associated 1 (non-protein coding)

Alias symbols: LINC00178; CUDR; UCAT1; onco-lncRNA-36

Alias names: long intergenic non-protein coding RNA 178; cancer up-regulated drug resistant

RefSeq ID: NR_015379

LncBook ID: [https://bigd.big.ac.cn/lncbook/transcript?transid=HSALNT0256354 HSALNT0256354]

===Characteristics===
[[File: UCA1 Transcript.jpg|right|thumb|400px|'''Full-length ''UCA1'' cDNA and gene structure''' <ref name="ref2" />.]]
''UCA1'' is three exon long noncoding RNA that is located on human chromosome 19p13.12. ''UCA1''  is spliced and polyadenylated <ref name="ref2" />. Multiple isoforms of ''UCA1'' exist (1.4kb, 2.2kb and 2.7kb). The 2.2kb isoform contains an extended final exon compared to the 1.4kb isoform while the 2.7kb isoform has not been characterised <ref name="ref1" /><ref name="ref2" />. Sequence is mainly repeat elements. Initiates in an LTR, exons sample pieces of a HERV-H element and longer 2.2kb isoform contains a second LTR at the 3' end <ref name="ref2" />.

===Function===
Functions of 1.4kb and 2.2kb isoforms have been investigated, both appear to promote and enhance tumourigenesis <ref name="ref1" /><ref name="ref3" />. Over-expression of 1.4kb isoform enhanced tumorigenic behavior of bladder cancer cells in-vitro and in-vivo. Increased drug resistance and cell motility <ref name="ref1" />. Overexpression of 2.2kb isoform caused resistance to drug induced apoptosis, potentially by downregulation of caspase 3 levels <ref name="ref3" />. 2.2kb isoform promoted transformation in-vitro, possibly because resistance to apoptosis is an important step in tumourigenesis <ref name="ref3" />.

''UCA1'' modulate ''MDR1'' by a model system of leukemia cell lines with a gradual increase of MDR1 expression and IM resistance. ''UCA1'' competes with 3' UTR of MDR1 mRNA for common miR-16 to regulate MDR1 expression. Overexpression of UCA1 increased MDR1 expression to promote Imatinib (IM) resistance of chronic myeloid leukemia (CML) cells <ref name="ref4" />.

Enhanced expression of ''UCA1'' is involved in cancer metastasis tongue squamous cell carcinoma <ref name="ref6" />.

''UCA1'' improve the cell migration, invasion and induce cisplatin resistance <ref name="ref9" />.

overexpression of ''UCA1'' promotes osteosarcoma initiation and progression <ref name="ref10" />.

''UCA1'' promotes hepatocellular carcinoma progression through inhibition of miR-216b by FGFR1/ERK signaling pathway <ref name="ref11" />.

===Expression===
Widely expressed in embryonic development and placenta <ref name="ref1" /><ref name="ref2" />. Low or no expression in normal adult tissue but highly upregulated in cell lines resistant to drug induced apoptotic cell death and bladder, colon and lung cancer <ref name="ref1" /><ref name="ref2" />, <ref name="ref3" />. 

''UCA1'' is upregulated in tongue squamous cell carcinomas and renal cell carcinoma <ref name="ref5" /><ref name="ref7" />.

Elevated expression of ''UCA1'' is found in ovarian cancer tissues <ref name="ref9" />.

''UCA1'' is overexpressed in osteosarcoma <ref name="ref10" />.

High expressed in gastric cancer<ref name="ref12" />.
{| class='wikitable' style="text-align:center"
|-
! | Experiment
! | Forward primer
! | Reverse primer
|-
| rowspan="1"|RT-PCR
| | 5′‐CTCTCCATTGGGTTCACCATTC‐3′
| | 5′‐GCGGCAGGTCTTAAGAGATGAG‐3′<ref name="ref1" />
|-
| rowspan="1"|PCR
| |  5′‐CGGGATCCTGACATTCTTCTGGACAATGAG‐3′
| | 5′‐CCGGAATTCGCATATTAGCTTTAATGTAGGTGGC‐3′<ref name="ref1" />
|-
| rowspan="1"|Real Time PCR
| | 5′‐GACCCTACCCGGTCATTTATAG‐3′
| | 5′‐CTGATGGGCATGGCTTTATTC‐3′<ref name="ref4" />
|-
|}
[[File: UCA1.jpg|right|thumb|400px|'''''UCA1'' expression is  promoted proliferation potential of BLS‐211 cells in vitro and in vivo''' <ref name="ref1" />.]]

===Conservation===
Found in humans. May be present in other primates but not in more distantly related mammals <ref name="ref1" />

===Disease===
* Bladder cancer <ref name="ref1" />
* Hepatocellular carcinoma <ref name="ref11" />
* Osteosarcoma <ref name="ref9" />
* Overian Cancer <ref name="ref10" />
* Pancreaticobiliary maljunction <ref name="ref8" />
* Renal cell carcinoma <ref name="ref7" />
* Tongue squamous cell carcinomas <ref name="ref5" /><ref name="ref6" />
* gastric cancer<ref name="ref12" />

==Labs working on this lncRNA==
* Department of Clinical Laboratory, Zhongshan Hospital of Xiamen University , Xiamen, Fujian Province, China.<ref name="ref4" />
* Department of Cardiothoracic Surgery, The Affiliated Dongnan Hospital of Xiamen University , Xiamen, Fujian Province, China.<ref name="ref4" />
* Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.<ref name="ref7" />
* Department of Urology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.<ref name="ref7" />
* The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.<ref name="ref7" />
* Department of Gynecology, Shaannxi Provincial People’s Hospital, Xi’an 710068, China.<ref name="ref9" />
* Department of Pediatrics, Second Affiliated Hospital of Xi’an Jiaotong University College of Medicine, Xi’an 710054, China.<ref name="ref9" />
* Center for Clinical Laboratory, Shaanxi Provincial People’s Hospital, Xi’an 710068, China.<ref name="ref9" />
* Central Laboratory, Shaanxi Provincial People’s Hospital, Xi’an 710068, China.<ref name="ref9" />

==References==
<references>
<ref name="ref1"> Wang F, Li X, Xie XJ, Zhao L, & Chen W. UCA1, a non‐protein‐coding RNA up‐regulated in bladder carcinoma and embryo, influencing cell growth and promoting invasion[J]. FEBS letters. 2008, 582(13):1919-1927.
</ref>(1)
<ref name="ref2"> Wang XS, Zhang Z, Wang HC, Cai JL, Xu Q W, Li MQ et al. Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma[J]. Clinical cancer research. 2006, 12(16):4851-4858.
</ref>(2)
<ref name="ref3"> Tsang W P, Wong T W L, Cheung A H H, et al. Induction of drug resistance and transformation in human cancer cells by the noncoding RNA CUDR[J]. Rna. 2007.
</ref>(3)
<ref name="ref4"> Xiao Y, Jiao C, Lin Y, Chen M, Zhang J, Wang J et al. lncRNA UCA1 contributes to imatinib resistance by acting as a ceRNA against miR-16 in chronic myeloid leukemia cells[J]. DNA and cell biology. 2017, 36(1):18-25.
</ref>(4)
<ref name="ref5"> Fang Z, Wu L, Wang L, Yang Y, Meng Y & Yang H. Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis[J]. Oral surgery, oral medicine, oral pathology and oral radiology. 2014, 117(1):89-95.
</ref>(5)
<ref name="ref6">  Yang QQ, Deng YF. Long non-coding RNAs as novel biomarkers and therapeutic targets in head and neck cancers[J]. International journal of clinical and experimental pathology. 2014, 7(4):1286.
</ref>(6)
<ref name="ref7"> Li Y, Wang T, Li Y, Chen D, Yu Z, Jin L et al. Identification of long-non coding RNA UCA1 as an oncogene in renal cell carcinoma[J]. Molecular medicine reports. 2016, 13(4):3326-3334.
</ref>(7)
<ref name="ref8"> Kaneko K, Ito Y, Ono Y, Tainaka T, Tsuchiya H, Shimoyama Y, et al. Gene expression profiling reveals upregulated UCA1 and BMF in gallbladder epithelia of children with pancreaticobiliary maljunction[J]. Journal of pediatric gastroenterology and nutrition. 2011, 52(6):744-750.
</ref>(8)
<ref name="ref9"> Wang F, Zhou J, Xie X,  Hu J, Chen L, Hu Q et al. Involvement of SRPK1 in cisplatin resistance related to long non-coding RNA UCA1 in human ovarian cancer cells[J]. Neoplasma. 2015.
</ref>(9)
<ref name="ref10"> Li W, Xie P, Ruan W. Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis[J]. Journal of bone oncology. 2016, 5(2):80-85.
</ref>(10)
<ref name="ref11"> Wang F, Ying HQ, He BS,  Pan YQ, Deng QW, Sun HL et al. Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway[J]. Oncotarget. 2015, 6(10):7899.
</ref>(11)
<ref name="ref12"> Cao WJ, Wu HL, He BS, et al. Analysis of long non-coding RNA expression profiles in gastric cancer[J]. World J Gastroenterol, 2013, 19: 3658-3664.</ref>(12)

</references>

===Sequence=== 
>gi|652995|ref| NR_015379.3|  Homo sapiens urothelial cancer associated 1 (UCA1), long non-coding RNA <dnaseq>TGACATTCTTCTGGACAATGAGTCCCATCATCTCTCCACCATGCACCTTGTGACTCCCTCCTCTGCTGAC
AACAGATAACCACCTTTAACTGTAACTTTCCACAGCCTACCCCAGCCCTATAAAGCTGCCCCTCTCCTAT
CTCCCTTCGCTGACTCTCTTTTCAGACTCAGCCCACTTGCACCCAAGTGAATTAACAGCCTTGTTGCTCA
CACAAAGCCTGTTTAGGTGGTCTTCTATACGGACATGCTTGACACTTGGTGCCAAAATCTGGGCCAGGGG
GACTCCTTCGTGAGACCGGCCCCCTGTCCTGGCCCTCATTCCGTGAAGAGATCCACCTGCGACCTCGGGT
CCTCAGACCAGCCCAAGGAACATCTCACCAATTTCAAATCGGATCTCCTCGGCTTAGTGGCTGAAGACTG
ATGCTGCCCGATCGCCTCAGAAGCCCCTTGGACCATCACAGATGCCGAGCTTCGGGTAACTCTTACGGTG
GAGGATTCCCAGCCATATGAAGACACCCTAGCTGGACGATCAGTCCTTGTCAAAAGTCTGACCCCTCAAA
CTCTACAGCCTCAATGGACCAGACCCTACCCGGTCATTTATAGCACACCAACTGCCGTCCATCTGCAGGA
CCCTCTCCATTGGGTTCACCATTCCAGAATAAAGCCATGCCCATCAGACAGCCAGCTTGATCTCTCCTCT
TCCTCCTGGAAGCCACAAGATTAGGCCGAGAGCCGATCAGACAAACAACCTACAACCCTTAAGCTCCTGG
CAGCGCCCAGCCAAGGCCATGCTTCCATGCAACACTCCTTCCAAATGGCCATCCCAGCATGCTTCCAAGC
AGGCTTCATCCGTTCCTCTGGACCCTCATCTCTTAAGACCTGCCGCCTATAAAAAGGATTATATCTTGAG
ACCCTATCCTCTAAAATTTTTTCCACACCCAAAACAAAAAATCTCTGGGTCAAAAGTCTAAAACGCTTAG
GCTGGCAACCATCAGATCCTTGCCCATGGTGTCCTCAAGCCTACTCTCATGAAATGGACAACAGTACACG
CATATGGGGCCAGTTCCACATATTTGGCAACCAGACCAGCATCCAGGACAACACAAAGTATGTTGTTTGT
TGTTAGAGGGCTTGGGACATTTCACTCTTTGCCAGCCTCAGCTTAATCCAGGAGACAAAGATTATTTTCC
TTATTATCTCTTCTGCATAGGATCTGCAATCAGAACTATTGAACTTCTCCATTCAGACCGCCACTCACAC
CTATGGGAAAAGGGTAATGTATCATCGGCTTAGCAACAGGGAATACTATTCGTATGATGGAAAATGGGGA
CAAAAGGCTTTGGTACATAAAACATTATTCCTTCCTTGGCCTAAAAACTCATCGCCACCTACATTAAAGC
TAATATGCCTGATTACTGTTTTTAGAGAACTTATTTTATTAGGGCAGTTCCAAGCTCAAAAATACGCTAA
CTGGCACCTTGTTAGCTACATAAAAATGCACCCTAGACCCGAAACTTACTAGACTCATTATAAAATTTTC
TTTAAGGTGTCCACGCAGTCCCTGGTCACACTTGAAGCAGTCCGGAGAAATATCAGCCCTACCCCAGTAA
TCCCCAGAAGGAACTTACACTTTTTTTTAATCTTTTCCTACAACTTCATATTTTATAAATAAAAAGACAA
AAATGTCAGGCCTGTGAGCTGAAGCTTAGCCATTGTAACCCCTGTGACCTGCACATATCCGTCCAGGTGG
CCTGCAGGAGCCAAGAAGTCTGGAGCAGCCGAAAAACCACAAAGAAGTGAAACAGCCAGTTCCTGCCTTA
ACTAATTAACCCACCTTACGACATTCCACCATTATGACTTGTCCACCATTATGACTTGTTCCTGCCCTGC
CCCAACTGATCAATCAACCCTGTGACATTCTTCTCCTGGACAATGAGTCCCATCATCTCTCCACCATGCA
CCTTGTGACCCCCTCCTCTGCTGAGGATAACCACCTTTAACTGTAACTTTCCACGCCTACCCAAGCCCTA
TAAAGCTGCCCCTCTCCTATCTCCCTTCACTGACTCTCTTTTCGGACTCAGCCCACTTGCACCCAAGTGA
ATTAACAGCCTTGTTGCTCACACAAAGCCTGATTGGGTGTCTTCTATACGGACACGCGTGACAGGAACCT
CAACCCAAAGGCAGTCTGATGAGGTGTCTAAGATAAAAGTAGCGGCACAAAGGCTTTTGTAAACAGAGGC
GTTTCATGTGGTTTTCCTTTCCTTTCCTTATATGTGAAAAGGTGACAGAAAAGAAATCTTCCTAAAAGAG
TCAG</dnaseq>