Difference between revisions of "LUNAR1"

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(Created page with "Please input one-sentence summary here. ==Annotated Information== ===Transcriptomic Nomeclature=== Please input transcriptomic nomeclature information here. ===Function=== P...")
 
(Annotated Information)
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===Transcriptomic Nomeclature===
 
===Transcriptomic Nomeclature===
 
Please input transcriptomic nomeclature information here.
 
Please input transcriptomic nomeclature information here.
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===Name===
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LUNAR1: leukemia-induced noncoding activator RNA
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===Characteristics===
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LUNAR1 is localized in the nucleus, and displays high correlation with IGF1R (insulin-like growth factor receptor 1).
  
 
===Function===
 
===Function===
Please input function information here.
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LUNAR1 functions as an enhancer in human T cell acute lymphoblastic leukemia (T-ALL). It is required for efficient T-ALL growth in vitro and in vivo due to its ability to enhance IGF1R mRNA expression and sustain IGF1 signaling.
 
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The specific enrichment of LUNAR1 at the IGF1R enhancer and LUNAR1 promoter has been observed. LUNAR1 RNA recruits RNAP2 and Mediator to sustain full activation of the IGF1R promoter.
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===Regulation===
 
===Regulation===
Please input regulation information here.
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LUNAR1 is directly controlled by the Notch1/Rpbjk activator complex. There is a highly active enhancer in the last intron of neighboring gene ''IGF1R''. This intronic enhancer, which is occupied by NOTCH1, Mediator subunit MED1, histone acetyltransferase P300, RNAPolII and the histone reader BRD4, activates the transcription of LUNAR1.
  
 
===Expression===
 
===Expression===
Please input expression information here.
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{| class='wikitable' style="text-align:center"
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|-
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! | Primer
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! | Forward primer
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! | Reverse primer
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|-
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| rowspan="1"|RT-PCR
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| | 5'-GGAGGCTGAGGCCGCCTGTT-3'
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| | 5'-AGGCTGCAGGGGAACAGGTCTT-3'
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|}
  
===Allelic Information and Variation===
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===Sequence===
Please input allelic information and variation information here.
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>LUNAR1 hg19 chr15:99,557,680-99,585,143
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AGGAATGGGAGAGGGAGGAGGACGCCAGGGGCACAGGGACAGCCTCCGGGGCGGGGCGCGGCGCCAGCCTAGCCCGAGGATGGAGGCTGAGGCCGCCTGTTGAGTCACAGTTTCCCTGTGATGCTCATTTTAACCAGATGGCACTTGCTTAGCAGTTTGGAGGAGAAGACCTGTTCCCCTGCAGCCTTCCATGATTAGACCTCAACTCATGAGAACTCTTGCCTTGCTCACAAGTGGGAGTAAATGCTCATAGCTCTAGAAATCTCCCAGGAACCAGACATCACGGAAACTAGAAAGGCTAAGGGAGTCCAATCTTCCTCTAAATTGGCCAACAGTAGAGTGGTGGAAAGAGAACAAGCTGCAAACCAGACAGACCCAAGCTTGAATCTCACCTCTGCCAAGCTACCAGAGCTCAACAAACTACAGACCACGGGCCAAATCTGACCCACCGCTGCTTTTGTAAATAAAACTTTATTGGAAAAAAAAAAAAA
  
===Evolution===
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===Labs working on this lncRNA===
Please input evolution information here.
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* Howard Hughes Medical Institute, Laura and Isaac Perlmutter Cancer Center, and Helen L. and Martin S. Kimmel Center for Stem Cell Biology
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.

Revision as of 17:14, 6 August 2014

Please input one-sentence summary here.

Annotated Information

Transcriptomic Nomeclature

Please input transcriptomic nomeclature information here.

Name

LUNAR1: leukemia-induced noncoding activator RNA

Characteristics

LUNAR1 is localized in the nucleus, and displays high correlation with IGF1R (insulin-like growth factor receptor 1).

Function

LUNAR1 functions as an enhancer in human T cell acute lymphoblastic leukemia (T-ALL). It is required for efficient T-ALL growth in vitro and in vivo due to its ability to enhance IGF1R mRNA expression and sustain IGF1 signaling. The specific enrichment of LUNAR1 at the IGF1R enhancer and LUNAR1 promoter has been observed. LUNAR1 RNA recruits RNAP2 and Mediator to sustain full activation of the IGF1R promoter.

Regulation

LUNAR1 is directly controlled by the Notch1/Rpbjk activator complex. There is a highly active enhancer in the last intron of neighboring gene IGF1R. This intronic enhancer, which is occupied by NOTCH1, Mediator subunit MED1, histone acetyltransferase P300, RNAPolII and the histone reader BRD4, activates the transcription of LUNAR1.

Expression

Primer Forward primer Reverse primer
RT-PCR 5'-GGAGGCTGAGGCCGCCTGTT-3' 5'-AGGCTGCAGGGGAACAGGTCTT-3'

Sequence

>LUNAR1 hg19 chr15:99,557,680-99,585,143 AGGAATGGGAGAGGGAGGAGGACGCCAGGGGCACAGGGACAGCCTCCGGGGCGGGGCGCGGCGCCAGCCTAGCCCGAGGATGGAGGCTGAGGCCGCCTGTTGAGTCACAGTTTCCCTGTGATGCTCATTTTAACCAGATGGCACTTGCTTAGCAGTTTGGAGGAGAAGACCTGTTCCCCTGCAGCCTTCCATGATTAGACCTCAACTCATGAGAACTCTTGCCTTGCTCACAAGTGGGAGTAAATGCTCATAGCTCTAGAAATCTCCCAGGAACCAGACATCACGGAAACTAGAAAGGCTAAGGGAGTCCAATCTTCCTCTAAATTGGCCAACAGTAGAGTGGTGGAAAGAGAACAAGCTGCAAACCAGACAGACCCAAGCTTGAATCTCACCTCTGCCAAGCTACCAGAGCTCAACAAACTACAGACCACGGGCCAAATCTGACCCACCGCTGCTTTTGTAAATAAAACTTTATTGGAAAAAAAAAAAAA

Labs working on this lncRNA

  • Howard Hughes Medical Institute, Laura and Isaac Perlmutter Cancer Center, and Helen L. and Martin S. Kimmel Center for Stem Cell Biology

You can also add sub-section(s) at will.

Labs working on this lncRNA

Please input related labs here.

References

Please input cited references here.