Long-term functional maintenance of primary human hepatocytes in vitro
| Title | Long-term functional maintenance of primary human hepatocytes in vitro |
|---|---|
| Description | The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening. |
| Organism | Homo sapiens |
| Data Type | Transcriptomic |
| Data Accessibility | Open-access |
| BioProject | PRJCA008650 |
| Release Date | 2023-12-16 |
| Submitter | Hongkui Deng (hongkui_deng@pku.edu.cn) |
| Organization | Peking University |
| Submission Date | 2022-03-14 |
The data cannot be downloaded as it has not yet been registered in the Human Genetic Resource Management Platform of MOST.
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX001033-20-01 | Batch123_RNA-seq_FPKM | 1 | Transcriptomic | 7.1 MB | xlsx | 0 | Unavailable |
| OMIX001033-20-02 | 24h-Cutured_hepatocytes_FPKM | 1 | Transcriptomic | 736.0 KB | xlsx | 0 | Unavailable |
| OMIX001033-20-03 | B1B2_RNA-seq_counts | 1 | Transcriptomic | 4.1 MB | xlsx | 0 | Unavailable |