OMIX

Transcriptomic change of hematopoietic stem cells isolated from bone marrow of 2D2 mice

OMIX001112

1Summary
Title Transcriptomic change of hematopoietic stem cells isolated from bone marrow of 2D2 mice
Description HSC were sorted from femur bone marrow of 2D2 TCR (TCR-MOG) transgenic and wild type mice. Cells were directly sorted into Trizol LS (ThermoFisher)-containing tubes, and total RNA was then extracted. Each mRNA was hibridized with two ~50 base probes in the hybidize solution. The reporter probe carries the signal, the capture probe allows the complex to be immobilized for data collection. After hybridization, excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartrige. Sample cartriges are placed in the Digital Analyzer. Color codes on the surface of the cartrige are counted and tabulated for each target mRNA. Data were processed on the nSolver software. Data were normalized to the 14 reference genes including positive and negative controls and housekeeping genes.
Organism Mus musculus
Data Type -
Data Accessibility Open-access
BioProject PRJCA007865
Release Date 2022-05-09
Submitter Qiang Liu (qliu@tmu.edu.cn)
Organization Tianjin Medical University General Hospital
Submission Date 2022-04-29
2Files & Download

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OMIX001112-51-01 Transcriptomic change of hematopoietic stem cells isolated from bone marrow of 2D2 mice 8 RNA Microarray 99.5 KB zip 0

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