LINE-1 insulates chromatin in a transcription-dependent manner
| Title | LINE-1 insulates chromatin in a transcription-dependent manner |
|---|---|
| Description | We uncover a class of L1s that enrich transcribing RNA polymerase IIs (Pol IIs), express L1 chimeric transcripts and insulate chromatin into topologically associating domains (TADs). We term these L1s 'the L1 insulators'. The insulating activity of L1s is inhibited by the nuclear matrix-associated protein Scaffold Attachment Factor B (SAFB). Knockout of SAFB increased Pol II binding at L1 insulators, and consequently strengthened TAD boundary formation. This effect was abolished by acute inhibition of Pol II transcription, indicating that the TAD boundary formation at L1s is dependent on ongoing transcription. Intriguingly, L1 insulators are characterized by enhancer-like chromatin signatures, and can affect gene transcription over long genomic distances. |
| Organism | Homo sapiens |
| Data Type | Other Type of Genomic Data |
| Data Accessibility | Open-access |
| BioProject | PRJCA005250 |
| Release Date | 2024-04-23 |
| Submitter | Nian Liu (liunian88@tsinghua.edu.cn) |
| Organization | Tsinghua University |
| Submission Date | 2022-07-20 |
HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client).
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX001330-02 | ChIP_seq_processed_Data | 71 | Other Type of Genomic Data | 13.64 GB | tar | 0 | |
| OMIX001330-03 | ChIP_seq_processed_Data_2 | 4 | Other Type of Genomic Data | 744.97 MB | tar | 0 | |
| OMIX001330-04 | ChIP_seq_processed_Data_3 | 2 | Other Type of Genomic Data | 438.13 MB | tar | 0 |