Branched-chain Amino Acid Aminotransferase-1 Regulates Self-Renewal and Pluripotency of Mouse Embryonic Stem Cells through Ras Signaling
| Title | Branched-chain Amino Acid Aminotransferase-1 Regulates Self-Renewal and Pluripotency of Mouse Embryonic Stem Cells through Ras Signaling |
|---|---|
| Description | In order to figure out the possible regulator which maintains the mouse Embryonic stem cells pluripotency and self-renewal, we performed RNA-seq in WT (cultured in normal medium with 2i and lif) and differential (cultured in the medium lacking of 2i and lif for 7 days) E14 cells (in three replicates, using Illumina HiSeq-2000). By analyzing the RNA-seq data, we found Bcat1 was highly expressed in WT E14 cells and decreased in differential E14 cells, and further studies showed that Bcat1 is essential for maintaining mESCs self-renewal and pluripotency. Next, we want to figure out how Bcat1 functions in mESCs. Mouse Embryonic stem cells -E14 mRNA of wild type (WT) and Bcat1-/- were generated by deep sequencing, in two replicates, using Illumina HiSeq-2000. |
| Organism | Mus musculus |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Open-access |
| BioProject | PRJCA011583 |
| Release Date | 2022-09-02 |
| Submitter | GIBH Biomedical Data & HPC (sciencedb@gibh.ac.cn) |
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission Date | 2022-09-01 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX001736-01 | omix-data | 7 | Expression Profiling by NGS | 3.9 MB | zip | 0 |