A distal enhancer maintaining Hoxa1 expression orchestrates retinoic acid-induced early ESCs differentiation
| Title | A distal enhancer maintaining Hoxa1 expression orchestrates retinoic acid-induced early ESCs differentiation |
|---|---|
| Description | Retinoic acid (RA) induces rapid differentiation of ESCs, partly by activating expression of the transcription factor Hoxa1, which regulates downstream target genes that promote ESCs differentiation. However, mechanisms of RA-induced Hoxa1 expression and ESCs early differentiation remain largely unknown. Here, we identify a distal enhancer interacting with the Hoxa1 locus through a long-range chromatin loop. Enhancer deletion significantly inhibited expression of RA-induced Hoxa1 and endoderm master control genes such as Gata4 and Gata6. Transcriptome analysis revealed that RA-induced early ESCs differentiation was blocked in Hoxa1 enhancer knockout cells, suggesting a requirement for the enhancer. Restoration of Hoxa1 expression partly rescued expression levels of ~40% of genes whose expression changed following enhancer deletion, and ~18% of promoters of those rescued genes were directly bound by Hoxa1. |
| Organism | Mus musculus |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Open-access |
| BioProject | PRJCA011597 |
| Release Date | 2022-09-03 |
| Submitter | GIBH Biomedical Data & HPC (sciencedb@gibh.ac.cn) |
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission Date | 2022-09-02 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX001746-01 | omix-data | 3 | Expression Profiling by NGS | 1.2 MB | zip | 0 |