Development of multiplex drop-off digital PCR assays for hotspot mutation detection of KRAS, NRAS, BRAF and PIK3CA in the plasma of colorectal cancer patients
| Title | Development of multiplex drop-off digital PCR assays for hotspot mutation detection of KRAS, NRAS, BRAF and PIK3CA in the plasma of colorectal cancer patients |
|---|---|
| Description | We developed novel multiplex drop-off digital PCR (MDO-dPCR) assays, by combining amplitude-/ratio-based multiplexing with drop-off/double drop-off strategies, which allow for detection of at least 69 most frequent hotspot mutations in all four genes with only three reactions. We assessed the analytical performance of the assays using synthetic oligonucleotides, further validated on plasma cfDNA samples from a large cohort of CRC patients and compared with next generation sequencing (NGS) data. |
| Organism | Homo sapiens |
| Data Type | Other Type of Clinical information |
| Data Accessibility | Open-access |
| BioProject | PRJCA015451 |
| Release Date | 2023-03-10 |
| Submitter | Huiqin Jiang (jiang.huiqin@zs-hospital.sh.cn) |
| Organization | Zhongshan Hospital, Fudan University |
| Submission Date | 2023-03-09 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX003268-01 | clinical data | 106 | Other Type of Clinical information | 90.8 KB | xlsx | 0 |