Comprehensive analysis of disulfidptosis-related genes reveals the effect of disulfidptosis in ulcerative colitis
| Title | Comprehensive analysis of disulfidptosis-related genes reveals the effect of disulfidptosis in ulcerative colitis |
|---|---|
| Description | An appropriate amount of sample was added to 400 microliters of precooled (4°C) methanol-water (v/v=4:1, containing 0.1% formic acid and 1 mM BHT, containing internal standard). Two steel beads were added, and the mixture was placed in a freezer at -20 °C for 2 min, then was vortexed for 1 min, sonicated in an ice-water bath for 10 min, and centrifuged for 10 min . 300 microliters of supernatant was evaporated. Then, 300 microliters of precooled methanol-water was added to the residue of the supernatant and repeat above step once time. The supernatant was resolubilized with 200 microliters of 95% aqueous acetonitrile (containing L-2-chlorophenylalanine), vortexed for 30 seconds, sonicated for 5 min in an ice-water bath, and centrifuged for 5 min. The supernatant was drawn up by a syringe, filtered by using a hydrophilic syringe with a 0.22 micrometers filter, transferred to a brown injection vial, and analyzed by LC-MS. The target metabolites were quantitatively and qualitatively detected by LC-MS. |
| Organism | Homo sapiens |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA020659 |
| Release Date | 2023-10-23 |
| Submitter | Huixian Song (shx502375150@163.com) |
| Organization | The First Affiliated Hospital of Kunming Medical University, Kunming, China |
| Submission Date | 2023-10-21 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX005124-01 | Homo sapoens | 12 | Metabolome Data by Mass Spectrometry (MS) | 317.3 KB | zip | 0 |