The gut microbiota-bile acid axis mediates the impairment of intestinal homeostasis by aspirin
| Title | The gut microbiota-bile acid axis mediates the impairment of intestinal homeostasis by aspirin |
|---|---|
| Description | C57BL/6J mice intestinal organoids were treated with different kinds of bile acids. Briefly, Secondary organoids were treated with CDCA (20 µM), 7-keto-LCA (20 µM) or UDCA (20 µM) for 5 days and next-generation sequencing conducted. Heatmap of changes in stem cell gene signature (Lgr5-Ascl2) in secondary C57BL/6J mouse organoids after the indicated treatments. Three representative replicates are show. Total RNA of organoids was extracted as described and RNA quality was confirmed using the Agilent 2100 Bioanalyzer. RNA-seq libraries were prepared from three biological replicates for each experimental condition and sequenced on an Illumina HiSeq 2500, 4000, or NextSeq500 using barcoded multiplexing and a 100-bp read length. Image analysis and base calling were done with Illumina CASAVA-1.8.2. The quality of the reads was assessed with fastqc. Reads were mapped against the reference genome and transcript annotation (GRCm38.p6) using STAR. |
| Organism | Mus musculus |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Open-access |
| BioProject | PRJCA021393 |
| Release Date | 2023-11-20 |
| Submitter | Ting Li (li.ting@xjtu.edu.cn) |
| Organization | The First Affiliated Hospital of Xi'an Jiaotong University |
| Submission Date | 2023-11-18 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX005259-01 | FKPM | 12 | Expression Profiling by NGS | 9.4 MB | xlsx | 0 |