Single-Cell Analysis Reveals Macrophage-Driven T Cell Dysfunction in Severe COVID-19 Patients
| Title | Single-Cell Analysis Reveals Macrophage-Driven T Cell Dysfunction in Severe COVID-19 Patients |
|---|---|
| Description | In this study, we explored how macrophage-driven T cell dysfunction occurs in COVID-19 patients using single-cell analysis. We analyzed leukocytes from pleural effusion, sputum, and peripheral blood biopsies across varying disease severities. Our findings revealed a significant increase in T cell hyperactivation and exhaustion, particularly noticeable in pleural effusion, indicating immune dysregulation after SARS-CoV-2 infection. We identified a specific subset of CD14+ monocytes and macrophages showing M2 polarization, with heightened expression of IL10, CCL18, APOE, CSF1 (M-CSF), and CCL2 signaling pathways. Additionally, SARS-CoV-2-specific T cells were detected earlier in pleural fluid than in peripheral blood. Overall, our results suggest that severe SARS-CoV-2 infection induces immune dysregulation through promoting M2 macrophage polarization and subsequent T cell exhaustion. |
| Organism | Homo sapiens |
| Data Type | Expression Profiling by NGS |
| Data Accessibility | Open-access |
| BioProject | PRJCA024048 |
| Release Date | 2024-07-01 |
| Submitter | Jiekai Chen (chen_jiekai@gibh.ac.cn) |
| Organization | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
| Submission Date | 2024-03-05 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX005929-01 | Human PBMC single-cell transcriptome counts data | 1 | Expression Profiling by NGS | 952.5 KB | zip | 0 | |
| OMIX005929-02 | Human PFMC single-cell transcriptome counts data | 1 | Expression Profiling by NGS | 1.7 MB | zip | 0 | |
| OMIX005929-03 | Human sputum single-cell transcriptome counts data (sample 1) | 1 | Expression Profiling by NGS | 780.0 KB | zip | 0 | |
| OMIX005929-04 | Human sputum single-cell transcriptome counts data (sample 2) | 1 | Expression Profiling by NGS | 1.2 MB | zip | 0 | |
| OMIX005929-05 | Human sputum single-cell transcriptome counts data (sample 3) | 1 | Expression Profiling by NGS | 759.9 KB | zip | 0 | |
| OMIX005929-06 | Human sputum single-cell transcriptome counts data (sample 4) | 1 | Expression Profiling by NGS | 820.7 KB | zip | 0 | |
| OMIX005929-07 | Human sputum single-cell transcriptome counts data (sample 5) | 1 | Expression Profiling by NGS | 757.6 KB | zip | 0 | |
| OMIX005929-08 | Human sputum single-cell transcriptome counts data (sample 6) | 1 | Expression Profiling by NGS | 822.0 KB | zip | 0 | |
| OMIX005929-09 | Human sputum single-cell transcriptome counts data (sample 7) | 1 | Expression Profiling by NGS | 969.1 KB | zip | 0 | |
| OMIX005929-10 | Human sputum single-cell transcriptome counts data (sample 8) | 1 | Expression Profiling by NGS | 1002.2 KB | zip | 0 | |
| OMIX005929-11 | Human sputum single-cell transcriptome counts data (sample 9) | 1 | Expression Profiling by NGS | 1.1 MB | zip | 0 | |
| OMIX005929-12 | Human sputum single-cell transcriptome counts data (sample 10) | 1 | Expression Profiling by NGS | 787.6 KB | zip | 0 | |
| OMIX005929-13 | Human sputum single-cell transcriptome counts data (sample 11) | 1 | Expression Profiling by NGS | 607.0 KB | zip | 0 | |
| OMIX005929-14 | Human sputum single-cell transcriptome counts data (sample 12) | 1 | Expression Profiling by NGS | 778.7 KB | zip | 0 | |
| OMIX005929-15 | Human sputum single-cell transcriptome counts data (sample 13) | 1 | Expression Profiling by NGS | 649.1 KB | zip | 0 | |
| OMIX005929-16 | Human sputum single-cell transcriptome counts data (sample 14) | 1 | Expression Profiling by NGS | 606.8 KB | zip | 0 | |
| OMIX005929-17 | Human sputum single-cell transcriptome counts data (sample 15) | 1 | Expression Profiling by NGS | 743.1 KB | zip | 0 | |
| OMIX005929-18 | Human sputum single-cell transcriptome counts data (sample 16) | 1 | Expression Profiling by NGS | 586.0 KB | zip | 0 | |
| OMIX005929-19 | Human sputum single-cell transcriptome counts data (sample 17) | 1 | Expression Profiling by NGS | 617.1 KB | zip | 0 |