Integrated Proteomics and Metabolomics Analysis of Sclerosis-Related Proteins and Femoral Head Necrosis Following Internal Fixation of Femoral Neck Fractures
| Title | Integrated Proteomics and Metabolomics Analysis of Sclerosis-Related Proteins and Femoral Head Necrosis Following Internal Fixation of Femoral Neck Fractures |
|---|---|
| Description | Nexera ultra-high-performance liquid chromatograph (SHIMADZU, Kyoto, JAPAN) hyphenated with Q-Exactive high-resolution tandem mass spectrometer equipped with heated electrospray ionization (ESI) source (Thermo Fisher Scientific, Waltham, MA, USA) was used to analyze the metabolic profiling in both ESI positive and ESI negative ion modes. An ACQUITY UPLC HSS T3 columnwas employed in positive and negative modes. The binary gradient elution system consisted of (A) water (containing 0.1% formic acid, v/v) and (B) ACN (containing 0.1 % formic acid, v/v) and separation was achieved using the following gradient: 0.01 min, 5% B; 2 min, 5% B; 4 min, 30% B; 8 min, 50% B; 10 min, 80% B; 14 min, 100% B; 15 min, 100% B; 15.1 min, 5% and 16 min, 5% B. The flow rate was 0.35 mL/min, and the column temperature was 45.All the samples were kept at 10 during the analysis. |
| Organism | Homo sapiens |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA025591 |
| Release Date | 2024-07-01 |
| Submitter | Yang Liu (15525127791@163.com) |
| Organization | Second Hospital of Shanxi Medical University |
| Submission Date | 2024-04-25 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006331-01 | LM2021-24607 | 3 | Metabolome Data by Mass Spectrometry (MS) | 1.13 GB | rar | 0 | Controlled |