The mass spectrometry analysis of PARP1-associated proteins
| Title | The mass spectrometry analysis of PARP1-associated proteins |
|---|---|
| Description | After 48 hours of transfection, cells overexpressing Myc-PARP1 were harvested. Cells were lysed and immunoprecipitated with anti-Myc antibodies according to co-immunoprecipitation protocol. Then the immunoprecipitated proteins were subjected to Western blot. The bands corresponding to PARP1 were excised in gel digested with trypsin and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. MS experiments were performed on a nanoscale UHPLC system (EASY-Nlc1000 from Proxeon Biosystems, Odense, Denmark) connected to an Orbitrap Q-Exactive equipped with a nanoelectros pray source (Thermo Fisher Scientific, Bremen, Germany). The raw data were processed using Proteome Discoverer (PD, version 2.1), and MS/MS spectra were searched against the reviewed Swiss-Prot human proteome database. |
| Organism | Homo sapiens |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA026452 |
| Release Date | 2024-05-25 |
| Submitter | Hao Li (haoli@cmu.edu.cn) |
| Organization | China Medical University |
| Submission Date | 2024-05-24 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006504-01 | The mass spectrometry analysis of PARP1-associated proteins | 1 | Proteomic Data by Mass Spectrometry (MS) | 73.9 KB | zip | 0 |