The dysregulation of lipid metabolism mediated by PPAR / FABP1 / SCD1 signaling triggered ferroptosis involved radiation-induced liver damage
| Title | The dysregulation of lipid metabolism mediated by PPAR / FABP1 / SCD1 signaling triggered ferroptosis involved radiation-induced liver damage |
|---|---|
| Description | The analytical instrument used in this study was a Dionex U3000 UHPLC (ultra-high-performance liquid chromatography system) coupled with a QE Plus high-resolution mass spectrometer. For metabolite extraction, 20 μL of 2-chloro-l-phenylalanine (0.3 mg/mL) dissolved in methanol, serving as the internal standard, and 1 mL of a methanol:water mixture (4: 1=v: v) were added to each sample. The resulting mixture was then transferred to a 4 mL glass vial. Subsequently, 200 μL of trichloromethane were subsequently added to each aliquot to disperse the sample using a pipette. The cells were disrupted using an ultrasonic homogenizer at 500 watts for 6 min.All of the mixtures from each sample were transferred to 1.5 mL Eppendorf tubes and extracted by ultrasonication for 20 min in an ice water bath. The resulting extract was centrifuged at 13,000 rpm and 4 for 10 min. |
| Organism | Mus musculus |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA028542 |
| Release Date | 2024-07-28 |
| Submitter | KaiXiao Zhou (17712664003@163.com) |
| Organization | Soochow University |
| Submission Date | 2024-07-26 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006996-01 | LM2020-12942 | 19 | Metabolome Data by Mass Spectrometry (MS) | 3.93 GB | tar | 0 |